Inferring regulatory targets of transcription factors from simultaneous single cell measurements of intranuclear proteins and gene expression. Inferring regulatory targets of transcription factors from simultaneous single cell measurements of intranuclear proteins and gene expression

Simultaneous single cell measurements of nuclear proteins and RNA with inCITE-seq, with targeted protein measurements using DNA-conjugated antibodies. Single nuclei (n=10,014) of HeLa cells, either untreated or after TNFa treatment, were profiled with inCITE-seq targeting the transcription factor p65. Single nuclei (n=21,583) of the mouse hippocampus after PBS or kainic acid injection were profiled with inCITE-seq targeting TFs p65, c-Fos, and PU.1, as well as the RNA-binding protein NeuN. Overall design: Joint single cell measurements of targeted nuclear proteins and RNA with inCITE-seq.

本数据集基于inCITE-seq技术实现细胞核蛋白与RNA的同步单细胞检测，并采用DNA偶联抗体完成靶向蛋白定量。针对转录因子p65的靶向inCITE-seq分析共完成10014个海拉（HeLa）细胞单细胞核样本的测序分析，样本分为未处理组与肿瘤坏死因子α（TNFα）处理组；针对转录因子p65、c-Fos、PU.1及RNA结合蛋白NeuN的靶向inCITE-seq分析共完成21583个小鼠海马体单细胞核样本的测序分析，样本经磷酸盐缓冲液（PBS）或红藻氨酸（kainic acid）注射处理。实验整体设计：采用inCITE-seq技术完成靶向细胞核蛋白与RNA的联合单细胞检测。