Parcellation scheme of rat iso- and proisocortical areas in Waxholm space (v2)

The present dataset provides a three-dimensional (3D) map of the rat iso- and proisocortex anchored in the standardized coordinate system of the Waxholm rat brain space. The parcellation encompasses 53 distinct areas and is the result of a multimodal analysis based on the heterogeneous regional and laminar distribution patterns of cell bodies and of muscarinic acetylcholine M2 receptors visualized in 2D sections. The receptor autoradiographs of a rat brain serially sectioned in the coronal plane were 3D reconstructed and anchored in Waxholm space. The ensuing transformation matrices were then applied to the delineations of the identified areas on the corresponding 2D autoradiographs, resulting in the registration of the location and extent of each area to the 3D reference Waxholm Space of the rat brain. This novel parcellation scheme of the rat iso- and proisocortical areas provides a high-resolution reference resource for the architectonically informed analysis of in vivo data and computational modelling approaches of the rat brain. As a dynamic framework, the Waxholm Rat Brain Atlas can be expanded by data of e.g., further neurotransmitter receptor types and brain regions.

本数据集构建了锚定至瓦克斯霍尔姆大鼠脑空间(Waxholm rat brain space)标准化坐标系的大鼠同型皮层与前同型皮层三维（3D）图谱。该脑区分区涵盖53个独立亚区，其构建基于2D切片中可视化的细胞体与毒蕈碱型乙酰胆碱M2受体的异质性区域分布与层状分布模式，经由多模态分析完成。对经冠状面连续切片的大鼠脑受体放射自显影图像完成三维重建，并锚定至瓦克斯霍尔姆空间；随后将所得变换矩阵应用于对应2D放射自显影图像中已识别亚区的轮廓标注，最终实现各亚区位置与范围配准至大鼠脑三维参考瓦克斯霍尔姆空间。这套全新的大鼠同型皮层与前同型皮层分区方案，可为基于神经解剖构筑特征的大鼠脑在体数据分析与计算建模研究提供高分辨率参考资源。作为一套动态框架，瓦克斯霍尔姆大鼠脑图谱(Waxholm Rat Brain Atlas)可通过新增的神经递质受体类型与脑区数据进行扩展。