Deciphering the lncRNA and mRNA profiles of Min pig backfat after acute cold stress

Cold stress can promote fat metabolism and reduce fat deposition. The present study compared differentially expressed (DE) lncRNAs and mRNAs in the subcutaneous back fat of Min pigs subjected to 24 h acute cold stress. Transcriptome sequencing was used to identify DE lncRNAs and mRNAs. A lncRNA mRNA co expression network was constructed using the confirmed lncRNAs. GO and KEGG analyses were performed to investigate the biological processes involved in the response to acute cold stress. A total of 166 DE lncRNAs and 269 DE mRNAs were found. Several genes such as ANGPTL4, KLF11 and NUPR1 were related to lipid metabolism. Interferon stimulating genes (ISGS) such as RSAD2, ISG20, ISG15, DDX60, MX2, MX1 and IFI6 were all significantly up-regulated. In addition, genes related to blood flow, respiration, and oxidative stress were also up-regulated. Many terms related to defense response to a virus were found in the GO annotation. A lncRNA mRNA co expression network comprising 46 specific mRNAs and the top 10 up-regulated DE lncRNAs was constructed. According to the results, the interaction between lncRNAs and mRNAs was complex. This study provides genome-wide gene and lncRNA expression profiles in adipose tissue of pigs after acute cold stress.

寒冷应激可促进脂肪代谢并减少脂肪沉积。本研究对经历24小时急性寒冷应激的民猪皮下背脂组织中的差异表达（differentially expressed，DE）长链非编码RNA（long non-coding RNA，lncRNAs）与信使RNA（messenger RNA，mRNAs）开展对比分析，采用转录组测序技术鉴定上述差异表达的lncRNAs与mRNAs。利用经鉴定的lncRNAs构建lncRNA-mRNA共表达网络，并通过基因本体（Gene Ontology，GO）富集分析与京都基因与基因组百科全书（Kyoto Encyclopedia of Genes and Genomes，KEGG）通路富集分析，探究急性寒冷应激响应相关的生物学过程。本研究共鉴定得到166个差异表达lncRNAs与269个差异表达mRNAs；其中ANGPTL4、KLF11及NUPR1等多个基因与脂质代谢相关；RSAD2、ISG20、ISG15、DDX60、MX2、MX1及IFI6等干扰素刺激基因（interferon stimulating genes，ISGs）均显著上调表达。此外，与血流调节、呼吸过程及氧化应激相关的基因同样出现上调表达。在GO注释结果中，富集得到大量与病毒防御响应相关的功能术语。本研究构建了包含46个特异性mRNA与10个上调幅度最高的差异表达lncRNAs的lncRNA-mRNA共表达网络，分析结果显示lncRNAs与mRNAs之间的相互作用关系较为复杂。本研究提供了急性寒冷应激后猪脂肪组织中全基因组范围的mRNA与lncRNA表达谱。