Figure 2 Source Data

Figure 2. MCH Improves 3T3-L1 Pre-adipocyte Viability in Culture. 3T3-L1 preadipocytes were cultured for six days at the indicated doses in the presence (n=3) or absence (n=6) of 100 nM PMC-3881-PI, an MCHR1 antagonist. To assess cell viability, a Crystal Violet Assay was performed and the A570 was recorded. Average +/- SEM is graphed. Statistical significance determined using a Student’s T-test. * indicates p图2 黑色素浓缩激素（MCH）可提升培养环境中3T3-L1前脂肪细胞的存活率。将3T3-L1前脂肪细胞以指定剂量分别置于添加（样本量n=3）或不添加（样本量n=6）100 nM PMC-3881-PI（一种黑色素浓缩激素受体1（MCHR1）拮抗剂）的培养基中培养6天。为评估细胞存活率，采用结晶紫染色分析法（Crystal Violet Assay）进行检测并记录A570吸光度值。以平均值±标准误（SEM）绘制统计图。统计学显著性采用学生t检验（Student’s T-test）进行判定。*代表p值