H3K27me3 CUT&Tag in keloid derived fibroblasts with or without LSP1P5 knocked down. Homo sapiens

To investigate the influence of LSP1P5 on H3K27me3 levels at the CEBPA promoter region in keloid-derived fibroblasts (KDFs), LSP1P5 knockdown was conducted using antisense oligonucleotide (ASO) in KDFs from two individuals. Subsequently, CUT&Tag assays were performed on both the knockdown groups (ASO-1) and the control group (ASO-NC). It is worth noting that initially, three ASO sequences were designed to target LSP1P5, and the original sequences, referred to as ASO2, showed the most significant effect. Therefore, this group was selected for the CUT&Tag assays, and the raw data file was labeled as ASO2. However, during the preparation of the manuscript, the original sequence name ASO2 was adjusted to ASO-1 for consistency in the published document.

为探究LSP1P5对瘢痕疙瘩来源成纤维细胞（keloid-derived fibroblasts, KDFs）中CCAAT增强子结合蛋白α（CEBPA）启动子区域组蛋白H3第27位赖氨酸三甲基化（H3K27me3）水平的影响，本研究针对2名供体的瘢痕疙瘩来源成纤维细胞，采用反义寡核苷酸（antisense oligonucleotide, ASO）开展LSP1P5敲低实验。随后分别对敲低组与对照组（ASO-NC）进行CUT&Tag实验。值得注意的是，研究初期共设计3条靶向LSP1P5的ASO序列，其中原命名为ASO2的序列敲低效果最为显著，因此选取该组开展后续的CUT&Tag实验，对应原始数据文件标记为ASO2。但在稿件撰写阶段，为确保发表文档的命名一致性，将原序列名ASO2调整为ASO-1。