The manually curated PinNRC3 variant rescued Rpi-blb2/Prf/Rx-mediated cell death.

(A) Amino acid sequence comparison of SlNRC3, Petunia NRC3 from the genome database and curated version. The indel was manually curated using sequences from SlNRC3. (B) Cell death assays of PinNRC3 variants co-expressed with Rpi-blb2/AVRblb2, Pto/AvrPto, or Rx/CP in nrc2/3/4_KO N. benthamiana. The auto-activity analysis was done by expressing NRC3 variants alone in WT N. benthamiana. The dot plots represent cell death intensity quantified by UVP ChemStudio PLUS at 6 dpi. The line in the boxplots represents the medium, the box edges represent the 25th and 75th percentiles, and the whiskers extend to the most extreme data points no more than 1.5x of the interquartile range. Statistical differences between the negative control (EV) and tested groups were examined by paired Wilcoxon signed rank test (* = p < 0.05).
(JPF)

(A) 对SlNRC3、基因组数据库中的矮牵牛NRC3以及经人工校正的版本开展氨基酸序列比对。本次分析中的插入缺失变异（indel）系通过SlNRC3的序列经人工手动校正获得。
(B) 在nrc2/3/4基因敲除的本氏烟（Nicotiana benthamiana）中，将PinNRC3变体分别与Rpi-blb2/AVRblb2、Pto/AvrPto或Rx/CP共表达以开展细胞死亡测定；另通过在野生型（WT）本氏烟中单独表达NRC3变体完成自动活性分析。散点图展示了通过UVP ChemStudio PLUS在接种后6天（dpi）定量得到的细胞死亡强度。箱线图中，横线代表中位数，箱边对应第25和第75百分位数，须线延伸至不超过四分位距1.5倍的最极端数据点。本研究采用配对威尔科克森符号秩检验对阴性对照（空载EV）与各实验组间的统计学差异进行检验，其中*代表p < 0.05。
(JPF)