Secondary screen of YopE secretion inhibition by selected small molecules.

The secretion of YopE was measured in the supernatants of bacterial cell culture by ELISA using anti-YopE antibody as described under Materials and Methods. The data were collected 1 hr post-induction with EGTA. Inhibitor concentrations were 10 µM. Positive and negative controls correspond to the results obtained for co-solvent (DMSO) without and with EGTA, respectively. Error bars correspond to standard deviation of five measurements. Errors correspond to standard deviation of five measurements.按照《材料与方法》中所述实验流程，采用抗YopE抗体，通过酶联免疫吸附实验（ELISA）对细菌细胞培养上清液中的YopE分泌量进行定量检测。实验数据于EGTA诱导1小时后采集，所用抑制剂终浓度为10 µM。阳性对照与阴性对照分别对应仅添加助溶剂二甲基亚砜（DMSO）而未添加EGTA，以及同时添加助溶剂（DMSO）与EGTA的实验组检测结果。误差棒代表五次重复测量的标准偏差，所有误差均基于五次测量的标准偏差计算得到。