Additional file 1 of A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones

Additional file 1: Table S1. Cytokinin (CK) concentrations (pmol mL-1) detected by HPLC-(ESI+)MS/MS in the cell-free supernatants of 46 Methylobacterium strains cultured in vitro in the DSM125 minimal medium. CK production was tested in the early stationary phase (5 – 7 days). Bacterial CKs scanned for by HPLC-(ESI+)MS/MS include: free base, riboside and nucleotide forms of Dihydrozeatin (DZ), trans-Zeatin (tZ) cis-Zeatin (cZ), and Isopentenyladenine (iP), and 2-methylthio-Zeatin (2MeSZ), 2-methylthio-Isopentenyladenine (2MeSiP), 2-methylthio-Zeatin riboside (2MeSZR), 2-methylthio-Isopentenyladenosine (MeSiPR). Values are means ± SE of 3 replicates; n.d. – not detected. Table S2. Cytokinin (CK) concentrations (pmol mL-1) detected by HPLC-(ESI+)MS/MS in the cell-free supernatants of Methylobacterium oryzae - LMG23582(T) strains cultured in vitro in the DSM125 minimal medium suplemented with various concentration of methanol. CK production was tested in 5-day old cultures. Bacterial CKs scanned for by HPLC-(ESI+)MS/MS include: free base, riboside and nucleotide forms of Dihydrozeatin (DZ), trans-Zeatin (tZ) cis-Zeatin (cZ), and Isopentenyladenine (iP), and 2-methylthio-Zeatin (2MeSZ), 2-methylthio-Isopentenyladenine (2MeSiP), 2-methylthio-Zeatin riboside (2MeSZR), 2-methylthio-Isopentenyladenosine (MeSiPR). Values are means ± SE of 3 replicates; n.d. – not detected.附加文件1：表S1。本表收录了46株甲基杆菌属（Methylobacterium）菌株在体外培养于DSM125基础培养基后，其无细胞上清液中经高效液相色谱-电喷雾正离子模式串联质谱（HPLC-(ESI+)MS/MS）检测得到的细胞分裂素（Cytokinin, CK）浓度，单位为pmol mL⁻¹。细胞分裂素产量的检测于培养稳定早期（5~7天）开展。本次通过HPLC-(ESI+)MS/MS扫描检测的细菌细胞分裂素涵盖：二氢玉米素（Dihydrozeatin, DZ）、反式玉米素（trans-Zeatin, tZ）、顺式玉米素（cis-Zeatin, cZ）、异戊烯腺嘌呤（Isopentenyladenine, iP），以及2-甲硫基玉米素（2-methylthio-Zeatin, 2MeSZ）、2-甲硫基异戊烯腺嘌呤（2-methylthio-Isopentenyladenine, 2MeSiP）、2-甲硫基玉米素核苷（2-methylthio-Zeatin riboside, 2MeSZR）、2-甲硫基异戊烯腺苷（2-methylthio-Isopentenyladenosine, MeSiPR）的游离碱形式、核苷形式与核苷酸形式。所有数值为3次重复的平均值±标准误（SE）；n.d.表示未检出。 表S2。本表收录了水稻甲基杆菌（Methylobacterium oryzae）LMG23582(T)菌株在体外培养于添加不同浓度甲醇的DSM125基础培养基后，其无细胞上清液中经高效液相色谱-电喷雾正离子模式串联质谱（HPLC-(ESI+)MS/MS）检测得到的细胞分裂素（CK）浓度，单位为pmol mL⁻¹。细胞分裂素产量的检测于培养第5天开展。本次通过HPLC-(ESI+)MS/MS扫描检测的细菌细胞分裂素涵盖：二氢玉米素（DZ）、反式玉米素（tZ）、顺式玉米素（cZ）、异戊烯腺嘌呤（iP），以及2-甲硫基玉米素（2MeSZ）、2-甲硫基异戊烯腺嘌呤（2MeSiP）、2-甲硫基玉米素核苷（2MeSZR）、2-甲硫基异戊烯腺苷（MeSiPR）的游离碱形式、核苷形式与核苷酸形式。所有数值为3次重复的平均值±标准误（SE）；n.d.表示未检出。