A longitudinal study of Blastocystis in dairy calves from Maryland, USA using NGAS.

Next generation amplicon sequencing of 437 samples of fecal DNA from 30 calves with the goal of investigating the infection dynamics of Blastocystis as well as subtype prevalence and diversity. PCR was used to amplify the SSU rRNA gene to screen for Blastocystis and subtype determination was performed using amplicon sequencing on an Illumina MiSeq.

对来自30头小牛的粪便DNA样本的437个样本进行下一代扩增子测序，旨在研究 Blastocystis 的感染动态、亚型普遍性和多样性。采用PCR技术扩增SSU rRNA基因以筛选 Blastocystis，并通过Illumina MiSeq平台的扩增子测序进行亚型鉴定。