RNA-seq data LV

Total RNA was isolated from left ventricular tissues with Trizol reagents (Invitrogen) followed by purification with the RNeasy Mini kit including DNase I digestion (Qiagen). RNA sequencing was carried out by Novogene corporation on Illumina Hiseq platform. The original sequencing data were trimmed using FASTX and aligned to the reference genome using TopHat2. The differential expression analysis was performed using Cuffdiff software.

采用Trizol试剂（Invitrogen公司）从左心室组织中提取总RNA，随后使用包含脱氧核糖核酸酶I（DNase I）消化步骤的RNeasy Mini试剂盒（Qiagen公司）完成纯化。RNA测序由诺禾致源（Novogene）公司在Illumina Hiseq测序平台上开展。原始测序数据通过FASTX软件进行序列修剪，并利用TopHat2软件比对至参考基因组。差异表达分析采用Cuffdiff软件完成。