Molecular Cytogenetic Analysis of a Gliosarcoma with Osseous Metaplasia. Homo sapiens

Gliosarcoma, a rare glioblastoma variant, is composed of a glial and a mesenchymal component. Though the mesenchymal portion most commonly resembles a fibrosarcoma, other differentiation patterns have been observed. We present the first genomic characterisation (karyotyping followed by FISH and array-CGH analysis) of a gliosarcoma with osseous metaplasia. In addition to chromosomal changes often found in gliomas (+7,-10,-13, and -22), the tumour cells also harboured a hitherto unknown 3q21;21q21~22-translocation. As translocations are very rare in high-grade astrocytic tumours and have not formerly been seen in gliosarcomas, it is possible that the t(3;21) may be characteristic of gliosarcoma with osseous metaplasia. Overall design: Tumour sample analysed with control DNA (supplied by Agilent)

胶质肉瘤（gliosarcoma）作为一种罕见的胶质母细胞瘤亚型，由胶质成分与间叶成分共同构成。尽管其间叶成分最常表现为纤维肉瘤样形态，但已有研究观察到其他分化模式。本研究首次对伴骨化生的胶质肉瘤开展基因组特征分析，依次采用染色体核型分析、荧光原位杂交（FISH）及阵列比较基因组杂交（array-CGH）技术。除胶质瘤中常见的染色体改变（+7、-10、-13及-22）外，该肿瘤细胞还携带了一种此前未被报道的3q21;21q21~22易位。鉴于易位在高级别星形细胞肿瘤中极为罕见，且此前从未在胶质肉瘤中被发现，因此t(3;21)可能是伴骨化生的胶质肉瘤的特征性遗传学改变。实验整体设计：采用安捷伦（Agilent）提供的对照DNA对肿瘤样本进行分析。