Study on the response of bacterial functional potential on temperate translocation experiment in Qinling forest

Here is a soil translocation experiment to reveal the temperature response of microorganisms and used 16S rRNA gene amplicon sequences to generalize bacterial characteristics concerning species turnover and community assembly, then combining PICRUSt estimated metagenomes to predict microbial function. In low elevation, eight of these cores were reinstalled at the same site (hereafter referred to as L), and the other eight cores translocated to the high elevation (hereafter referred to as CL), which was called translocation cooling process. Similarly, in high elevation, eight of these cores were reinstalled at the same site (hereafter referred to as H), and the other eight cores translocated to the high elevation (hereafter referred to as WH), which was called translocation warming process. This study suggested that the short term temperature response of dormancy potential regulates bacterial species turnover through altering community assembly.

本研究开展土壤易位实验以揭示微生物的温度响应规律，采用16S rRNA基因扩增子序列（16S rRNA gene amplicon sequences）解析细菌在物种更替与群落构建方面的特征，并结合PICRUSt预测的宏基因组数据推演微生物功能。在低海拔样地中，8个土壤柱被保留在原点位（以下简称L组），剩余8个土壤柱被易位至高海拔样地（以下简称CL组），该处理称为易位降温过程。同理，在高海拔样地中，8个土壤柱被保留在原点位（以下简称H组），其余8个土壤柱被易位至低海拔样地（以下简称WH组），该处理称为易位增温过程。本研究表明，休眠潜能的短期温度响应通过改变群落构建过程调控细菌物种更替。