Finding two synergized compounds from Chinese herbal medicine in rheumatoid arthritis therapy. Finding two synergized compounds from Chinese herbal medicine in rheumatoid arthritis therapy

Purpose: To search candidate synergized compounds from Chinese herbal medicine used in RA therapy Methods: Candidate synergized compounds were searched based on multi-information integrative approaches. In vivo experiment in collagen induced arthritis rat was then carried out to evaluate the efficacy and synergism of compounds combination treatment. RNA sequencing and IPA were used to investigate the synergized mechanism of combination treatment. Results: We found a pair of compound combination which could alleviate the symptom of CIA rats. RNA sequencing and IPA analysis revealed that the synergistic mechanism of combination treatment was related to several canonical signaling pathways and key targets LTA, CD83 and SREBF1. Conclusion: A pair of compound combination has been found. RNA sequencing and IPA analysis revealed that the synergistic mechanism of combination treatment was related to several canonical signaling pathways and key targets. Overall design: CIA rats were treated with compound combination for 4 weeks. After sacrifice, the CD4+T lymphocytes from peripheral blood of rats were collected for further RNA sequencing.

研究目的：从类风湿关节炎（Rheumatoid Arthritis, RA）治疗用中草药中筛选候选协同化合物。
研究方法：采用多信息整合策略筛选候选协同化合物；随后构建胶原诱导性关节炎（Collagen Induced Arthritis, CIA）大鼠体内模型，评估化合物联合给药的疗效与协同效应；通过RNA测序（RNA sequencing）与IPA（Ingenuity Pathway Analysis）分析联合给药的协同作用机制。
研究结果：本研究筛选得到一组化合物组合，可有效缓解CIA大鼠的疾病症状；RNA测序与IPA分析显示，该联合给药的协同作用机制与多条经典信号通路及关键靶点LTA、CD83、SREBF1密切相关。
研究结论：本研究筛选得到一组化合物组合；RNA测序与IPA分析揭示，该联合疗法的协同作用机制与多条经典信号通路及关键靶点相关。
整体实验设计：对CIA大鼠施以化合物联合给药干预，持续4周；待大鼠处死后，采集其外周血中的CD4+T淋巴细胞，用于后续RNA测序实验。