Pedigreed Primate Embryonic Stem Cells Express Homogeneous Familial Gene Profiles

Pedigreed primate ESCs display homogeneous and reliable expression profiles. These similarities to mouse ESCs suggest that heterogeneities found among hESCs result from their disparate origins rather than intrinsic biological limitations with primate embryonic stem cells. Keywords: different nhpESC lines RNA extraction was performed from 10 nhpESC and hybridization on Affymetrix microarrays. To determine whether primate ESCs have intrinsic biological limitations compared with mouse ESCs [mESCs], we examined expression profiles and pluripotency of newly established pedigreed non-human primate ESC (nhpESCs). Ten pedigreed nhpESCs, seven full-siblings (fraternal quadruplets and fraternal triplets) and nine half-siblings were derived from 41 rhesus embryos Information about pedigrees may be found in Pedigree_Attributes.xls.具有完整谱系的灵长类胚胎干细胞（Embryonic Stem Cells, ESCs）展现出均一且稳定的表达谱。 这类细胞与小鼠胚胎干细胞（mouse Embryonic Stem Cells, mESCs）的相似性提示，人类胚胎干细胞（human Embryonic Stem Cells, hESCs）中观察到的细胞异质性源于其各异的起源，而非灵长类胚胎干细胞本身存在内在生物学局限。 关键词：不同非人灵长类ESCs细胞系；本研究从10株非人灵长类胚胎干细胞（non-human primate Embryonic Stem Cells, nhpESCs）中提取RNA，并在Affymetrix微阵列上完成杂交。 为探究相较于小鼠胚胎干细胞，灵长类胚胎干细胞是否存在内在生物学局限，我们对新构建的具有完整谱系的nhpESCs的表达谱与多能性进行了检测。本研究从41枚恒河猴胚胎中构建得到10株具有完整谱系的nhpESCs，其中7株为全同胞（包括异卵四胞胎与异卵三胞胎），另有9株为半同胞。 谱系相关详细信息可查阅Pedigree_Attributes.xls文件。