Basal-to-inflammatory transition enhances basal cell carcinoma (BCC) therapy resistance via crosstalk with a pro-inflammatory stromal niche [RNA-seq II]

The goal of these RNA-seq experiments was to determine transcriptomic gene expression changes caused by 7 days of Il1a and Osm combination treatment of mouse primary BCC tumor-derived organoids compared to PBS control Overall design: Mouse primary BCC tumor tissues were embedded as small pieces in basement membrane extract and grown in organoid medium for 7 days. Cells were treated with either PBS control or Il1 and Osm ligand combination for 7 days in organoid medium.

本RNA测序（RNA-seq）实验旨在确定经白细胞介素1α（Il1a）与制瘤素M（Osm）联合处理7天的小鼠原发性基底细胞癌（BCC）肿瘤来源类器官，与磷酸盐缓冲液（PBS）对照组相比的转录组基因表达变化。
实验设计概要：将小鼠原发性BCC肿瘤组织以小块形式包埋于基底膜提取物中，于类器官培养基中培养7天；随后分别使用PBS对照或Il1与Osm配体联合处理细胞，在类器官培养基中继续培养7天。