Preoperative exercise therapy triggers anti-inflammatory trained immunity of Kupffer cells through metabolic reprogramming. Preoperative exercise therapy triggers anti-inflammatory trained immunity of Kupffer cells through metabolic reprogramming

Single-cell transcriptomics revealed that transcriptomic profile is shifted towards anti-inflammatory in Kupffer cells after pre-operative exercise therapy in mice. Overall design: To investigate single cell transcriptomics on Kupffer cells, we obtained hepatic CD45+ leukocytes. Four-week exercise or sedentariness were performed, and liver tissue was harvested, followed by cell isolation, FACs sorting, and 10X Genomics Chromium Drop-Seq and library construction. Illumina Next-Seq sequencing of barcoded single nuclei libraries was performed and aligned to a pre-mrna genome with STAR/CellRanger. Gene/barcode matrices were merged and processed with the Seurat R package.

单细胞转录组学（single-cell transcriptomics）研究显示，小鼠接受术前运动疗法后，其库普弗细胞（Kupffer cells）的转录组谱向抗炎表型偏移。
整体实验设计：为探究库普弗细胞的单细胞转录组特征，本研究分离得到肝脏CD45阳性白细胞。对小鼠实施为期4周的运动训练或久坐饲养，随后采集肝脏组织，依次进行细胞分离、流式细胞分选、10X Genomics Chromium Drop-Seq及文库构建。对带有条形码标记的单细胞核文库采用Illumina Next-Seq进行测序，并通过STAR/CellRanger工具将测序数据比对至前mRNA基因组。最终通过Seurat R软件包对基因-条形码矩阵进行合并与数据分析。