Structural Biology at EMBL Grenoble, IBS-CIBB, IAB and ILL

This proposal clusters macromolecular crystallography (MX) and biological small angle X-ray scattering (bioSAXS) projects at the EMBL Grenoble, IBS-CIBB, IAB and ILL. It combines groups working on different topics with a major focus on cell signaling proteins, ubiquitination pathways, nucleic acid-protein complexes, sarcomere development, host-pathogen interactions and drug discovery. The BAG group has been quite productive this last year/reporting period, as summarized in the report, with many results still being further analysed and follow-up experiments planned. Most notably, the BAG has produced 7 PDB depositions, 1 SASDB deposition and 10 publications in peer reviewed journals. The proposers have long-standing experience in the use, as well as the development of beam lines for MX and bioSAXS, and have participated in successive successful BAG applications over the years, mostly at the ESRF. The BAG responsible is again Andrew McCarthy (EMBL Grenoble) for this application round.

本提案针对欧洲分子生物学实验室格勒诺布尔分部（EMBL Grenoble）、IBS-CIBB、IAB以及ILL的大分子晶体学（macromolecular crystallography, MX）与生物小角X射线散射（biological small angle X-ray scattering, bioSAXS）项目进行集群整合。该提案将研究主题各异的课题组进行统筹，核心聚焦于细胞信号通路蛋白、泛素化途径、核酸-蛋白复合物、肌节发育、宿主-病原体互作以及药物发现等研究方向。BAG组在过去一年/本次报告周期内成果斐然，如本报告所述，多项研究成果仍处于进一步分析阶段，后续跟进实验亦已完成规划。尤为突出的是，BAG组已完成7项蛋白质数据库（Protein Data Bank, PDB）数据提交、1项小角散射数据库（SASDB）数据提交，并在同行评议期刊发表学术论文10篇。本提案申请人在MX与bioSAXS光束线的使用及开发领域拥有长期丰富的经验，多年来多次参与欧洲同步辐射光源（European Synchrotron Radiation Facility, ESRF）的成功BAG申请项目。本次申请周期的BAG负责人仍为安德鲁·麦卡锡（Andrew McCarthy，EMBL Grenoble）。