Data_Sheet_1_Self-Assembled Thin-Layer Glycomaterials With a Proper Shell Thickness for Targeted and Activatable Cell Imaging.docx

The construction of targeted and activatable materials can largely improve the precision of disease diagnosis and therapy. However, the currently developed systems either target a transmembrane antigen or are activatable to release imaging and/or therapeutic reagents intracellularly. Here, we develop a simple thin-layer glycomaterial through the self-assembly between fluorescent glycoprobes, in which the carbohydrate-targeting reagent and the fluorophore are linked to each other by polyethylene glycol with a suitable chain length, and thin-layer manganese dioxide. The fluorogenic material developed is both capable of targeting a transmembrane glycoprotein receptor and fluorescently activatable by intracellular biothiols. The shell thickness of the material was determined to be important for achieving the biothiol-induced activation of fluorescence. This research might provide insight into the development of precision-enhanced self-assembled materials for disease theranostics.靶向可激活材料的构建，可大幅提升疾病诊断与治疗的精准度。然而，当前已开发的相关系统，要么仅靶向跨膜抗原（transmembrane antigen），要么仅可在细胞内被激活以释放成像试剂和/或治疗试剂。本研究通过荧光糖探针（fluorescent glycoprobes）与薄层二氧化锰（thin-layer manganese dioxide）的自组装，构建了一种简易薄层糖基材料；其中，糖类靶向试剂（carbohydrate-targeting reagent）与荧光团（fluorophore）通过链长适配的聚乙二醇（Polyethylene Glycol, PEG）相互连接。所开发的该荧光材料，既可靶向跨膜糖蛋白受体（transmembrane glycoprotein receptor），又可被细胞内生物硫醇（intracellular biothiols）激活以产生荧光信号。研究表明，该材料的壳层厚度对实现生物硫醇介导的荧光激活过程至关重要。本研究可为面向疾病诊疗一体化（theranostics）的精准化自组装材料开发提供新思路。