DataSheet4_NMR Metabolite Profiles of the Bivalve Mollusc Mytilus galloprovincialis Before and After Immune Stimulation With Vibrio splendidus.pdf

The hemolymph metabolome of Mytilus galloprovincialis injected with live Vibrio splendidus bacteria was analyzed by 1H-NMR spectrometry. Changes in spectral hemolymph profiles were already detected after mussel acclimation (3 days at 18 or 25 °C). A significant decrease of succinic acid was accompanied by an increase of most free amino acids, mytilitol, and, to a smaller degree, osmolytes. These metabolic changes are consistent with effective osmoregulation, and the restart of aerobic respiration after the functional anaerobiosis occurred during transport. The injection of Vibrio splendidus in mussels acclimated at 18°C caused a significant decrease of several amino acids, sugars, and unassigned chemical species, more pronounced at 24 than at 12 h postinjection. Correlation heatmaps indicated dynamic metabolic adjustments and the relevance of protein turnover in maintaining the homeostasis during the response to stressful stimuli. This study confirms NMR-based metabolomics as a feasible analytical approach complementary to other omics techniques in the investigation of the functional mussel responses to environmental challenges.

本研究采用氢核磁共振波谱法（1H-NMR），分析了注射活灿烂弧菌（Vibrio splendidus）的地中海贻贝（Mytilus galloprovincialis）的血淋巴代谢组。在贻贝经驯化培养（于18℃或25℃下培养3天）后，即可检测到其血淋巴光谱轮廓的变化。此时琥珀酸水平显著下降，同时多数游离氨基酸、贻贝醇以及少量渗透调节物质的水平升高，这类代谢变化与有效的渗透调节，以及运输过程中发生功能性无氧代谢后有氧呼吸的重启相一致。对在18℃下驯化的贻贝注射灿烂弧菌，会导致多种氨基酸、糖类及未归属化学物质的水平显著下降，且该变化在注射后24小时比12小时更为显著。相关热图分析表明，在应对胁迫刺激的过程中，贻贝存在动态代谢调节，且蛋白质周转在维持机体稳态中具有关键作用。本研究证实，基于1H-NMR的代谢组学技术是一种可行的分析手段，可与其他组学技术互补，用于探究贻贝对环境胁迫的功能性响应。