Wedelolactone facilitates the development of porcine early embryo by reducing the oxidative stress

Wedelolactone (WDL) is a couma ether compound extracted from the traditional Chinese medicinal plant, Eclipta prostrata. It is a natural polyphenol that exhibits a variety of pharmacological activities, such as anti-inflammatory, anti-free radical scavenging, and antioxidant activities in the bone, brain, and ovary. However, its effect on embryonic development remains unknown. The present study explored the influence of WDL supplementation on porcine oocytes culture in vitro, its underlying mechanisms, and the levels of Kelch-like ECH-associated protein 1/nuclear factor-erythroid 2-related factor 2/antioxidant response element. The results showed that WDL (2.5 nM) significantly increased the blastocyst formation rate, mitochondrial activity, and proliferation ability, while reducing the reactive oxygen species accumulation, apoptosis, and autophagy.魏德内酯（Wedelolactone，WDL）是一种从传统药用植物墨旱莲（Eclipta prostrata）中提取的香豆素醚类化合物。其属于天然多酚类物质，在骨骼、大脑及卵巢组织中可发挥抗炎、抗自由基清除、抗氧化等多种药理活性。但目前其对胚胎发育的影响仍未明确。本研究探究了外源性补充WDL对猪卵母细胞体外培养的影响、潜在作用机制，以及Kelch样ECH相关蛋白1（Kelch-like ECH-associated protein 1）、核因子红细胞2相关因子2（nuclear factor-erythroid 2-related factor 2）与抗氧化反应元件（antioxidant response element）的表达水平。结果显示，浓度为2.5 nM的WDL可显著提升囊胚形成率、线粒体活性与细胞增殖能力，同时降低活性氧蓄积、细胞凋亡及细胞自噬水平。