H3K27Me3 (Abcam2).BG3.BEAF_RNAi

modENCODE_submission_3679 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The goal of these experiments are to validate and confirm the locations of 125 chromosomal proteins across the Drosophila melanogaster genome. To do this, we are using RNAi to deplete individual non-histone chromosomal proteins in Drosophila BG3 and S2 tissue culture cells, and then using antibodies to perform Chromatin ImmunoPrecipitation (ChIP) using genomic tiling arrays. Comparison of a protein factor's binding profiles before and after depletion will increase the confidence of our predictions. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Cell Line: ML-DmBG3-c2; Tissue: CNS-derived cell-line; Developmental Stage: third instar larval stage; Genotype: y v f mal; Sex: Unknown; NUMBER OF REPLICATES: 4; EXPERIMENTAL FACTORS: Cell Line ML-DmBG3-c2; Antibody H3K27Me3 (Abcam2) (target is H3K27Me3); dsRNA (RNAi_reagent) CG10159_RNAi&oldid=39153

modENCODE_submission_3679 本提交源自Gary Karpen牵头的modENCODE项目。如需查看完整modENCODE项目列表，请访问http://www.genome.gov/26524648。

项目目标：本系列实验旨在验证并确认125种染色体蛋白在黑腹果蝇（Drosophila melanogaster）全基因组中的结合位点。具体实验方案为：在果蝇BG3与S2组织培养细胞中通过RNA干扰（RNA interference, RNAi）技术单独耗除非组蛋白染色体蛋白，随后使用抗体开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验，并结合基因组平铺阵列（genomic tiling array）进行检测。通过对比蛋白因子在RNA干扰前后的染色质结合谱，可提升结合位点预测结果的可信度。

数据使用条款与条件请参阅：http://www.genome.gov/27528022 与 http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。

实验类型：ChIP-chip（染色质免疫沉淀-芯片）

生物来源：
细胞系：ML-DmBG3-c2；
组织来源：中枢神经系统（CNS）来源细胞系；
发育阶段：三龄幼虫期；
基因型：y v f mal；
性别：未知；
生物学重复次数：4次；

实验因子：
细胞系：ML-DmBG3-c2；
抗体：H3K27Me3（Abcam2）（靶标为H3K27Me3）；
双链RNA（dsRNA，RNAi试剂）：CG10159_RNAi&oldid=39153