6S RNA mimics B-form DNA to regulate Escherichia coli RNA polymerase (MOLECULAR-CELL-D-17-01031). Chen, et al.

Files contain raw unprocessed data used in Chen et al. "6S RNA mimics B-form DNA to regulate Escherichia coli RNA polymerase". Please see paper for figure labels and legends as well as details on how experiments were performed.

Figure S1
-‘5labeled_footprinting’ and ‘3labeled_footprinting’ gel images are labeled in Figure S1B
-Lanes 1 and 2 of ‘transcription_assay_6S_deltaCS’ correspond to Figure S1C (left panel)
-Lanes 5 and 6 of ‘transcription_assay_6S_RNA_mutant’ correspond to Figure S1C (right panel)

Figure S5
-‘XL_test_copy’ is labeled in Figure S5A
-Boxed part of ‘gel3_XLhot_copy’ is represented and labeled in Figure S5B
-Boxed part of ‘PE_mapping_copy’ is represented and labeled in Figure S5C

Figure S6
-Boxed part of ‘stem_mutants_copy’ is represented and labeled in Figure S6A
-Boxed part of ‘G82_mutants_binding copy’ is represented and labeled in Figure S6B
-Boxed part of ‘binding_131_135_136_others_copy’ is represented and labeled in Figure S6C
-Lanes 1-12 of ‘Wt_FYWW_T7A1DS_T7A1bubble’ correspond to Figure S6E (top panel)
-Lanes 13-24 of ‘Wt_FYWW_T7A1DS_T7A1bubble’ correspond to Figure S6E (middle panel)
-‘Wt_FYWW_6S_deltaCS’ is represented in Figure S6E (bottom panel)

Figure 7
Gels used to generate Figure 7C are oriented to have the following loading ordering (left to right):
1) T7A1 – Wt SigS
2) T7A1 – SigS E308K/E315H
3) T7A1 – Wt Sig70
4) T7A1 – Sig70 K593E/H600E
5) DPS100 – Wt SigS
6) DPS100 – SigS E308K/E315H
7) DPS100 – Wt Sig70
8) DPS100 – Sig70 K593E/H600E
9) 6S RNA – Wt SigS
10) 6S RNA – SigS E308K/E315H
11) 6S RNA – Wt Sig70
12) 6S RNA – Sig70 K593E/H600E
*File names also denote the trial number.

Figure S7
Gels used to generate Figure S7B are oriented to have the following loading ordering (left to right):
1) Wt SigS
2) SigS L116F
3) SigS E217K
4) SigS G228D
5) SigS E308K
6) SigS E315H
7) Wt Sig70
8) Sig70 F401L
9) Sig70 K502E
10) Sig70 D513G
11) Sig70 K593E
12) Sig70 H600E
*File names also denote transcription template used and trial number.

本数据集包含Chen等人在论文《6S RNA模拟B型DNA以调控大肠杆菌RNA聚合酶》中使用的未经处理的原始实验数据。相关图片的标注、图例以及实验操作细节，请参见该论文原文。

### 补充图S1
- 图S1B中标注了5'端标记足迹法（5'labeled_footprinting）与3'端标记足迹法（3'labeled_footprinting）的凝胶电泳图谱
- `transcription_assay_6S_deltaCS`的第1、2泳道对应补充图S1C的左半部分
- `transcription_assay_6S_RNA_mutant`的第5、6泳道对应补充图S1C的右半部分

### 补充图S5
- 图S5A中标注了`XL_test_copy`
- 补充图S5B展示并标注了`gel3_XLhot_copy`的框选区域
- 补充图S5C展示并标注了`PE_mapping_copy`的框选区域

### 补充图S6
- 补充图S6A展示并标注了`stem_mutants_copy`的框选区域
- 补充图S6B展示并标注了`G82_mutants_binding copy`的框选区域
- 补充图S6C展示并标注了`binding_131_135_136_others_copy`的框选区域
- `Wt_FYWW_T7A1DS_T7A1bubble`的第1至12泳道对应补充图S6E的上半部分
- `Wt_FYWW_T7A1DS_T7A1bubble`的第13至24泳道对应补充图S6E的中半部分
- 补充图S6E的下半部分展示了`Wt_FYWW_6S_deltaCS`的实验结果

### 图7
用于生成图7C的凝胶电泳图谱的上样顺序（从左至右）如下：
1. T7A1 – 野生型西格玛因子S（SigS）
2. T7A1 – SigS E308K/E315H双突变体
3. T7A1 – 野生型西格玛因子70（Sig70）
4. T7A1 – Sig70 K593E/H600E双突变体
5. DPS100 – 野生型SigS
6. DPS100 – SigS E308K/E315H双突变体
7. DPS100 – 野生型Sig70
8. DPS100 – Sig70 K593E/H600E双突变体
9. 6S RNA – 野生型SigS
10. 6S RNA – SigS E308K/E315H双突变体
11. 6S RNA – 野生型Sig70
12. 6S RNA – Sig70 K593E/H600E双突变体
* 文件名同时标注了实验重复次数。

### 补充图S7
用于生成补充图S7B的凝胶电泳图谱的上样顺序（从左至右）如下：
1. 野生型SigS
2. SigS L116F突变体
3. SigS E217K突变体
4. SigS G228D突变体
5. SigS E308K突变体
6. SigS E315H突变体
7. 野生型Sig70
8. Sig70 F401L突变体
9. Sig70 K502E突变体
10. Sig70 D513G突变体
11. Sig70 K593E突变体
12. Sig70 H600E突变体
* 文件名同时标注了所用的转录模板以及实验重复次数。