Full-Length Transcriptome Analysis of the Pituitary of Pre-matured and matured female Japanese eel (Anguilla japonica)

Japanese eel (Anguilla japonica) is a migratory fish with high economic value. The gonads of eels cultured in captivity do not mature naturally, and under artificial ripening, their hypothalamic-pituitary-gonadal axis is activated and the maturation coefficient of the gonads is significantly increased. Previous studies on eel breeding have focused on reproductive physiological processes, with limited insight into the molecular mechanisms in the pituitary gland that influence ovarian development. To address these limitations, we used pituitary tissues of female Japanese eels as samples for Pacbio Iso-Seq and RNA-Seq combining analyses, selecting pre-matured (PP) and after-matured (AP) samples to investigate DEGs and signal pathways regulating gonadal development in the pituitary gland, respectively. Overall design: Full - length transcriptome sequencing of the pituitaries of pre-matured and matured Japanese eels were carried out by combining Illumina RNA - Seq and PacBio Iso - Seq technologies.

日本鳗鲡（Anguilla japonica）是具有极高经济价值的洄游性鱼类。人工养殖的鳗鲡性腺无法自然成熟，而在人工促熟条件下，其下丘脑-垂体-性腺轴（hypothalamic-pituitary-gonadal axis）被激活，性腺成熟系数显著升高。以往关于鳗鲡繁育的研究多聚焦于生殖生理过程，对垂体中影响卵巢发育的分子机制的解析较为有限。为弥补上述研究不足，本研究以雌性日本鳗鲡的垂体组织为样本，开展Pacbio Iso-Seq与RNA-Seq联合分析，选取成熟前期（PP）和成熟后期（AP）的样本，分别探究垂体中调控性腺发育的差异表达基因（differentially expressed genes, DEGs）及信号通路。实验设计：结合Illumina RNA-Seq与Pacbio Iso-Seq技术，对成熟前期和成熟日本鳗鲡的垂体组织开展全长转录组测序。