Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes. Lysine specific demethylase 1 inactivation enhances differentiation and promotes cytotoxic response when combined with all-trans retinoic acid in acute myeloid leukemia across subtypes

Combined treatment with all-trans retinoic acid and GSK2879552 results in synergistic effects on gene expression, cell proliferation, markers of differentiation, and, most importantly, cytotoxicity. Overall design: Gene expression analysis of DMSO, single and combination treatment (ATRA and GSK2879552) on 6 AML cell lines at two time-points with two replicates (paired end RNA-seq on 96 samples in total)

全反式维甲酸（all-trans retinoic acid, ATRA）与GSK2879552联合处理，在基因表达、细胞增殖、分化标志物以及最为关键的细胞毒性层面均展现出协同效应。整体实验设计：选取6株急性髓系白血病（Acute Myeloid Leukemia, AML）细胞系，对二甲基亚砜（Dimethyl Sulfoxide, DMSO）对照组、单药处理组与联合处理组（ATRA与GSK2879552）开展基因表达分析，设置两个采样时间点，每个处理设置两次生物学重复，共计96个样本进行双端RNA测序（paired end RNA-seq）。