Browning of White Adipose Tissue with Roscovitine Induces a Distinct Population of UCP1+ Adipocytes.. Mus musculus

Analysis of FACS sorted UCP1+ adipocytes shows that the mRNA signature of brite adipocytes is distinct from beige adipocytes, which arise through catecholamine signaling. Overall design: Total RNA samples of FACS-sorted adipocytes (3-4 each group) from UCP1/Tomato mice (8 to 10-week old) were analyzed by microarray at Boston University Microarray Resource Facility.

本研究针对经荧光激活细胞分选术（Fluorescence-Activated Cell Sorting，FACS）分离的解偶联蛋白1阳性（UCP1+）脂肪细胞开展分析，结果显示亮脂肪细胞（brite adipocytes）的mRNA表达特征与米色脂肪细胞（beige adipocytes）存在显著差异，后者通过儿茶酚胺信号通路分化形成。实验设计概述：采集8~10周龄UCP1/Tomato小鼠的脂肪细胞，经FACS分选后提取总RNA（每组3-4份），随后于波士顿大学微阵列资源中心通过微阵列技术完成转录组检测分析。