Secale cereale Lo7 assembly - additional Hi-C raw data

The data were used in the 2019 assembly of domesticated rye (Secale cereale), variety Lo7, described in Rabanus-Wallace et al. (2019). A detailed description of the assembly process is given in the Supplementary Materials. The basic assembly scaffolds were constructed from mate-paired, paired-end, and 10X Chromium linked read data, using the NRGene DeNovoMagic 3 pipeline. Scaffolds were then curated and arranged into pseudomolecules with the use of 10X Chromium data, BioNano Optical map alignments, Chromosome Sorted Shotgun data, genetic map positions lifted from the previous assembly of Bauer et al. (2017), and Hi-C conformation capture data.These accession contains Hi-C raw reads of Secale cereale and Secale strictum. PE-libraries (from DpnII digest) were sequenced using Illumina HiSeq2500.本数据集应用于2019年发布的栽培黑麦（Secale cereale）品种Lo7的基因组组装工作，相关内容由Rabanus-Wallace等人（2019）报道。 组装流程的详细说明见于补充材料。 研究依托NRGene DeNovoMagic 3分析管线，利用mate-paired（配对插入片段）、paired-end（双端）及10X Chromium连锁读长测序数据构建了基础组装支架（scaffolds）。 随后通过10X Chromium数据、BioNano光学图谱比对结果、染色体分选鸟枪法测序数据、从Bauer等人（2017）先前组装版本映射得到的遗传图谱位点，以及Hi-C构象捕获数据，对支架序列进行校正并排布为假染色体（pseudomolecules）。 本数据集包含栽培黑麦（Secale cereale）和硬黑麦（Secale strictum）的Hi-C原始测序读段。 该数据集的双端测序文库（经DpnII限制性内切酶酶切）采用Illumina HiSeq2500平台完成测序。