Gene expression profiling of mouse spleen XCR1+ DC at steady state, 3 hours after polyI:C or 12 hours after STAg i.v. injection

The goal of this experiment was to use global gene expression profiling to compare the global genetic reprogramming of spleen XCR1+ DC upon in vivo stimulation with a viral-type ligand, polyI:C which strongly induces type I interferons, versus with a ligand derived from an intracellular parasite which strongly induces IFN-g. DC were isolated as previously described (Robbins SH et al. Genome Biol. 2008. PMID: 18218067; Baranek T et al. Cell Host Microbe. 2012. PMID: 23084923), from the spleens of wild-type C57BL/6 mice 12 hours after i.v. injection of 20µg of STAg. DC subsets were sorted by flow cytometry according to the marker combinations described in the “characteristics: phenotype” field for each sample. The data for spleen XCR1+ DC isolated from untreated control animals or from mice that had been injected i.v. with 100µg polyI:C 3 hours before organ harvest have been previously published and are available in the GEO database GSE39556 series. The complete dataset representing: (1) the Samples corresponding to STAg stimulation and (2) the untreated control Samples and the Samples corresponding to polyI:C stimulation from Series GSE39556 (re-processed using RMA), is linked below as a supplementary file.

本实验采用全基因组表达谱分析技术，对比脾脏XCR1阳性树突状细胞（dendritic cell, DC）经两种配体体内刺激后的全局遗传重编程模式：其一为可强效诱导I型干扰素产生的病毒类配体polyI:C，其二为可强效诱导干扰素-γ（IFN-γ）产生的胞内寄生虫来源配体。树突状细胞的分离流程参照既往发表的方法（Robbins SH等, *Genome Biol*, 2008, PMID: 18218067；Baranek T等, *Cell Host Microbe*, 2012, PMID: 23084923）：采集经静脉注射20μg STAg后12小时的野生型C57BL/6小鼠脾脏，随后按照每份样本"特征：表型"字段中规定的标记物组合，通过流式细胞术分选出对应树突状细胞亚群。此前已发表的数据集包含未经处理的对照组小鼠，以及组织采集前3小时经静脉注射100μg polyI:C的小鼠的脾脏XCR1阳性树突状细胞样本，该数据集现已上传至GEO数据库GSE39556系列。本完整数据集涵盖两部分内容：(1) STAg刺激组样本；(2) GSE39556系列中的未处理对照组样本与polyI:C刺激组样本（已通过RMA算法重新处理），现已作为补充文件附于下文。