Supporting data for "A Database of Restriction Maps to Expand the Utility of Bacterial Artificial Chromosomes"

While Bacterial Artificial Chromosomes were once a key resource for the genomic community, they have been obviated, for sequencing purposes, by long-read technologies. Such libraries may now serve as a valuable resource for manipulating and assembling large genomic constructs. To enhance accessibility and comparison, we have developed a BAC restriction map database. Using information available from the National Center for Biotechnology Informations cloneDB ftp site, a database containing the restriction maps for both uniquely placed and insert-sequenced BACs from 11 libraries covering available restriction enzyme recognition sequences was constructed. Along with the database, a set of python functions are available for reconstructing the database and more easily accessing information within. This data is valuable for researchers simply using BACs, as well as working with larger sections of the genome in terms of synthetic genes, large-scale editing, and mapping.

细菌人工染色体（Bacterial Artificial Chromosomes，BAC）曾是基因组学领域的核心资源之一，但就测序应用而言，长读长测序技术已使其被取代。此类文库如今可作为操作与组装大型基因组构建体的宝贵资源。为提升可访问性与可比较性，我们构建了一套细菌人工染色体限制性酶切图谱数据库。我们依托美国国家生物技术信息中心（National Center for Biotechnology Information，NCBI）cloneDB的FTP站点公开信息，构建了涵盖11个文库中唯一定位及插入片段已测序的细菌人工染色体的限制性酶切图谱数据库，覆盖所有可用的限制性内切酶识别序列。本数据库配套提供了一套Python函数工具集，可用于数据库的重建以及更便捷地获取库内信息。该数据集不仅对仅使用细菌人工染色体的研究人员具有应用价值，也可助力开展合成基因、大规模基因组编辑与基因组图谱绘制等涉及大片段基因组操作的研究工作。