Sirtuin-1 is required for proper trophoblast differentiation and placental development

In this study we identified that Sirt1 is important for mouse trophoblast stem cell (TSC) differentiation. The transcriptome of wild-type and Sirt1-null TSC were analyzed to identify dysregulation of signaling pathways. In order to identify gene expression changes in TSC in the absence of Sirt1, we performed genome-wide expression profiling of Wild-type and Sirt1-null TSCs derived from a littermate pair of embryos on the 129S1/SvlmJ genetic background.本研究发现，沉默信息调节因子1（Sirt1）对小鼠滋养层干细胞（trophoblast stem cell, TSC）的分化至关重要。为鉴定信号通路的失调情况，本研究对野生型与Sirt1基因缺失型TSC的转录组进行了分析。为明确Sirt1缺失时TSC内的基因表达变化，本研究对取自129S1/SvlmJ遗传背景下同窝胚胎的野生型与Sirt1基因缺失型TSCs开展了全基因组表达谱分析。