Gene expression signatures of spleen-derived and bone marrow-derived CD11b+ cells in high-fat diet (HFD)-fed mice. Gene expression signatures of spleen-derived and bone marrow-derived CD11b+ cells in high-fat diet (HFD)-fed mice

To further search for potential gene targets which are involved in the pathological process of atherosclerosis, we have employed whole genome microarray expression profiling as a discovery platform to identify the expression of genes related to lipid metabolism. The CD11b+ cells were isolated from spleen and bone marrow of mice by magnetic beads, and four genotype mice of HDC-/-, STAT4-/-, STAT6-/- and WT were examined in this experiment. The mice were divided into 2 groups: 1. control group 2. HFD group. Mice in control group were given a normal diet; mice in HFD group were fed with high-fat diet for 3 months. The data showed hundreds of up-regulated or down-regulated genes in HFD-fed mice, which were functionally connected with cholesterol synthesis and reverse cholesterol transport. Overall design: The gene expression was measured in spleen-derived and bone marrow-derived CD11b+ cells of mice. Mice were divided into control and HFD groups. There were five mice in each group.

为进一步筛选参与动脉粥样硬化病理进程的潜在基因靶点，本研究采用全基因组微阵列表达谱作为发现平台，以鉴定与脂质代谢相关的基因表达情况。本实验通过磁珠分选法从小鼠脾脏与骨髓中分离CD11b阳性细胞（CD11b+），并对HDC-/-、STAT4-/-、STAT6-/-及野生型（WT）四种基因型小鼠开展检测。将小鼠分为两组：1. 对照组；2. 高脂饮食组（HFD）。对照组小鼠饲喂正常饲料，高脂饮食组小鼠连续饲喂高脂饲料3个月。数据分析显示，高脂饮食喂养的小鼠体内存在数百个上调或下调基因，这些基因的功能与胆固醇合成及胆固醇逆转运密切相关。实验设计概述：本研究检测了小鼠脾脏及骨髓来源的CD11b阳性细胞中的基因表达水平。小鼠被分为对照组与高脂饮食组，每组各包含5只小鼠。