Next Generation Sequencing of tomato germplasm for cold tolerance and yield studies. Next Generation Sequencing of tomato germplasm for cold tolerance and yield studies

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goal of this study is genome wide association mapping for cold tolerance and yield studies in tomato (Solanum spp.) by phenotyping the genotypes under six diffrent temporal arrangments. Also for conducting quantitative reverse transcription polymerase chain reaction (qRT–PCR) assay of cold tolerant genes. Overall design: 50 samples

研究目的：下一代测序（Next-generation sequencing, NGS）已彻底革新了细胞通路的系统级分析。本研究的目标包含两项：其一，通过在六种不同时间排布条件下对番茄（Solanum spp.）的基因型进行表型分型，开展该作物耐寒性的全基因组关联定位与产量相关研究；其二，对耐寒基因实施定量逆转录聚合酶链反应（quantitative reverse transcription polymerase chain reaction, qRT–PCR）检测。整体实验设计：共包含50个样本。