Plasmodium falciparum and P. vivax amplicon sequencing

To understand transmission patterns and differences in infection prevalence in the Chittagong Hill Tracts of Bangladesh, residents were enrolled into a cross sectional survey and blood samples were screened for any Plasmodium spp. infection using qPCR. Genotyping by size-polymorphic markers and amplicon deep-sequencing was applied to assess relationships among infections within and between ethnic groups. To understand transmission patterns in Ethiopia, malaria patients were enrolled at health centers throughout the country. Dried blood spots were screened for P. falciparum infection using PCR. Genotyping by size-polymorphic markers and amplicon deep-sequencing was applied to assess relationships among infections within and between sites. Sample IDs beginning with GPAT_ were used for the study in Bangladesh. Sample IDs beginning with E_ were used for the study in Ethiopia.为探究孟加拉国吉大港山区（Chittagong Hill Tracts）的疟疾传播模式及感染率差异，本研究招募当地居民开展横断面调查，采用定量聚合酶链式反应（quantitative polymerase chain reaction, qPCR）对血液样本进行疟原虫属（Plasmodium spp.）感染筛查；通过尺寸多态性标记基因分型及扩增子深度测序技术，分析不同族群内部及族群间的感染关联特征。为探究埃塞俄比亚的疟疾传播模式，本研究在该国各地医疗中心招募疟疾患者，采用聚合酶链式反应（polymerase chain reaction, PCR）对干血斑样本进行恶性疟原虫（Plasmodium falciparum, P. falciparum）感染筛查，并通过尺寸多态性标记基因分型及扩增子深度测序技术，分析不同采样点内部及采样点间的感染关联特征。孟加拉国研究使用的样本ID以GPAT_为前缀，埃塞俄比亚研究使用的样本ID以E_为前缀。