DNA methylation analysis of radiation-induced skin fibrosis of rats

This study aims to compared the genome-wide DNA methylation status in radiation-induced fibrotic skin and adjacent normal tissues of rats by methylated DNA immunoprecipitation sequencing (MeDIP-Seq). Male Sprague-Dawley (SD) rats (4 weeks old) were irradiated with a single 45-Gy dose of irradiation was administered to the treatment area at a rate of 750 cGy/min using a 6-MeV electron beam accelerator (Clinac 2100EX, Varian Medical Systems, Inc., CA). Skin tissues from nonirradated skin areas and irradietd areas were collected and subjected to DNA methylation analysis by MeDIP. Male Sprague-Dawley (SD) rats (4 weeks old) were irradiated with a single 45-Gy dose of irradiation was administered to the treatment area at a rate of 750 cGy/min using a 6-MeV electron beam accelerator (Clinac 2100EX, Varian Medical Systems, Inc., CA). Skin tissues from nonirradated skin areas (NC) and irradietd areas were collected and subjected to DNA methylation analysis by MeDIP.

本研究旨在通过甲基化DNA免疫沉淀测序（MeDIP-Seq），对比大鼠放射性纤维化皮肤与邻近正常组织的全基因组DNA甲基化状态。选取4周龄雄性斯普拉格-道利（SD）大鼠，采用6兆电子伏特电子直线加速器（Clinac 2100EX，瓦里安医疗系统公司，加利福尼亚州），以750厘戈瑞每分钟的剂量率单次向治疗区域给予45戈瑞的放射线照射。收集未照射皮肤区域与照射区域的皮肤组织，通过MeDIP进行DNA甲基化分析。重复上述实验流程：选取4周龄雄性斯普拉格-道利（SD）大鼠，采用6兆电子伏特电子直线加速器（Clinac 2100EX，瓦里安医疗系统公司，加利福尼亚州），以750厘戈瑞每分钟的剂量率单次向治疗区域给予45戈瑞的放射线照射；收集未照射皮肤区域（NC）与照射区域的皮肤组织，通过MeDIP进行DNA甲基化分析。