Phylosymbiosis of Rodent in the Great Rift Valley Raw sequence reads. Phylosymbiosis of Rodent in the Great Rift Valley

Host phylogeny and the environment play vital roles in shaping animal microbiomes. However, the effects of these variables on the diversity and richness of the gut microbiome in different bioclimatic zones remain underexplored. The objective of this study is to investigate the effects of host phylogeny and bioclimatic zone on the diversity and composition of the gut microbiota of two heterospecific rodent species, Acomys cahirinus and the house mouse Mus musculus, in three bioclimatic zones of the African Great Rift Valley (GRV). We confirmed the host phylogeny using the D-loop sequencing method and analyzed the core microbiome and the influence of host phylogeny and bioclimatic zone parameters on the microbiome of the rodent gut using high-throughput amplicon sequencing of 16S rRNA gene fragments. We found that compared to the differences between species, the bioclimatic zone had a considerably greater effect on the gut microbiota composition. Sequencing was performed at the Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna under the project ID JMF-2106-01.

宿主系统发育与环境在塑造动物微生物组的过程中发挥着关键作用。然而，目前针对不同生物气候区中，宿主与环境两类变量对肠道微生物组多样性与丰富度的影响，相关研究仍有待深入探索。本研究旨在探究非洲大裂谷（GRV）三个生物气候区内，宿主系统发育与生物气候区对两种异种啮齿动物——埃及刺鼠（Acomys cahirinus）与小家鼠（Mus musculus）肠道微生物群多样性与组成的影响。本研究采用D环（D-loop）测序法验证宿主系统发育关系，并通过16S rRNA基因片段的高通量扩增子测序技术，分析啮齿动物肠道核心微生物组，以及宿主系统发育、生物气候区参数对肠道微生物组的影响。研究结果显示，相较于物种间的差异，生物气候区对肠道微生物群组成的影响更为显著。本研究的测序工作由维也纳医科大学与维也纳大学联合微生物组中心依托项目编号JMF-2106-01完成。