Transcriptomic profiling of adipose tissue in periparturient dairy cows

Periparturient cows rely on adipose tissue fatty acid reserves released by lipolysis to offset the negative energy balance induced by physiological changes related to parturition and the onset of lactation. However, lipolysis causes inflammation and structural remodeling in excess predisposes cows to disease. The objective of this study was to determine effects of the periparturient period on the transcriptomic profile of AT using NGS RNAseq mRNA profile of 12 mammary glands from cows before and at two time points after parturition. Subcutaneous adipose biopsies were collected on day -11, day 6 and day 13 relative to parturition围产期奶牛依赖脂解（lipolysis）作用释放的脂肪组织（adipose tissue）脂肪酸储备，以抵消由分娩（parturition）与泌乳启动相关生理变化所诱导的能量负平衡（negative energy balance）。然而，过度脂解会引发炎症反应与结构重塑，使奶牛更易罹患疾病。本研究旨在明确围产期对脂肪组织转录组谱（transcriptomic profile）的影响，采用NGS RNA-seq mRNA测序技术，对12份采集于分娩前及分娩后两个时间点的奶牛乳腺组织样本进行转录组分析。研究人员在相对于分娩的第-11天、第6天及第13天采集了皮下脂肪活检（subcutaneous adipose biopsies）样本。