Expression data of health individuals and newly diagnosed multiple myeloma

Multiple myeloma is a multi-stage disease. Based on its laboratory and clinical presentation it can be summarized as MGUS, smoldering myeloma, and multiple myeloma. Previous studies have shown that the expression levels of miRNAs in different stages of the disease are different. This study analyzes the expression levels of miRNAs in healthy individuals and myeloma serum exosomes. We used microarrays to detail the miRNAs expressionin between 12 healthy individuals and 12 multiple myeloma and identified distinct classes of dys-regulated genes. Collecting multiple myeloma patients and healthy individuals peripheral blood and serum and extract exosomes, extract exosome RNA, and hybridize on Affymetrix microarrays.

多发性骨髓瘤（Multiple myeloma）是一种多阶段疾病。根据其实验室与临床表现，可将其划分为意义未明单克隆免疫球蛋白血症（MGUS）、冒烟型多发性骨髓瘤（smoldering myeloma）以及多发性骨髓瘤。既往研究显示，该疾病不同阶段的微小RNA（microRNA，miRNA）表达水平存在差异。本研究针对健康个体与骨髓瘤患者血清外泌体（exosomes）中的miRNA表达水平开展分析。我们通过基因芯片（microarrays）详细分析了12名健康个体与12名多发性骨髓瘤患者的miRNA表达情况，并鉴定出多类异常调控基因（dys-regulated genes）。本研究收集多发性骨髓瘤患者与健康个体的外周血及血清，分离外泌体并提取外泌体RNA，随后在Affymetrix基因芯片上完成杂交实验。