Comparaison of left and right cervical motoneurons transcriptome. Mus musculus

Left and Right phrenic nerves, which innervate the left and right diaphragm muscles, exhibit different innervation patterns. This left/right (L/R) asymmetry is established at the onset of innervation by a developmental program that requires Nodal. Phenotype analysis suggests that the cervical motoneurons, which innervate the diaphragm, have a L/R imprint that contributes to set the L/R asymmetries of innervation. We used microarray to analyze the expression profile of left and right cervical motoneurons before diaphragm innervation Overall design: Left and right cervical motoneurons pools were collected from E11.5 HB9::GFP mice embryos. Left and right pools were collected from the same sections to allow paired analysis. The three embryos were from the same litter to minimize expression changes due to variation of the developmental stages.支配左右膈肌的左、右膈神经，二者的神经支配模式存在显著差异。这种左右（L/R）不对称性在神经支配起始阶段，由依赖于Nodal的发育程序所确立。表型分析结果表明，支配膈肌的颈运动神经元携带有左右侧印记，该印记参与确立神经支配的左右不对称性。本研究采用基因芯片(microarray)技术，对膈肌神经支配前的左右侧颈运动神经元的表达谱进行了分析。总体实验设计：从发育第11.5天（E11.5）的HB9::GFP转基因小鼠胚胎中收集左右侧颈运动神经元池。为开展配对分析，左右侧神经元池取自同一脊髓切片。实验所用的3枚胚胎均来自同窝，以尽可能降低因发育阶段差异引发的基因表达变化。