Transcriptional profiles of Drosophila melanogaster testes and seminal vesicles/testes terminal epithelium.

Testes and testes terminal epithelium/seminal vesicles were dissected and separated from 4-5 day old Oregon-R-modENCODE Drosophila melanogaster males. RNA-sequencing was then performed on these two distinct biological structures. Overall design: Testes and testes terminal epithelium/seminal vesicles were dissected and separated from 4-5 day old Oregon-R-modENCODE Drosophila melanogaster males on two consecutive days resulting in two biological replicates. Total RNA was immediately extracted and used to prepare poly-A RNA-seq libraries. Single-end 50 bp sequencing was performed on all four samples and mapped to the Flybase Drosophila melanogaster release 6.06 genome.

我们从4-5日龄的Oregon-R-modENCODE品系黑腹果蝇（Drosophila melanogaster）雄虫中解剖分离出睾丸及睾丸末端上皮/精囊两种组织，随后对二者开展RNA测序（RNA-sequencing）。整体实验设计：连续两天分别从4-5日龄的上述品系黑腹果蝇雄虫中解剖分离目标组织，以此获得两份生物学重复样本。实验中即刻提取总RNA，用于构建多聚腺苷酸富集的RNA测序文库。对全部四个样本实施单端50bp测序，并将测序序列比对至Flybase发布的黑腹果蝇参考基因组版本6.06。