Data_Sheet_1_Association between TMPRSS2 rs2070788 polymorphism and COVID-19 severity: a case-control study in multiple cities of Iran.docx

IntroductionHost genetic variations have been identified as potential influencers of COVID-19 infection. This study aimed to examine the association between transmembrane serine protease type 2 (TMPRSS2) rs2070788 single nucleotide polymorphism (SNP) and the prognosis of COVID-19 in Iranian populations. MethodThis case-control study was performed on 756 COVID-19 patients and 59 healthy individuals across Iran. Clinical data, blood samples, and the presence of the TMPRSS2 rs2070788: G>A SNP were determined using T-ARMS-PCR. Additionally, serum levels of tumor necrosis factor α (TNF-α), C-reactive protein (CRP), interleukin-6 (IL-6), and IL-1β were evaluated in the collected blood samples. ResultsNo significant association was found between the genotypes and allele frequencies of TMPRSS2 rs2070788 SNP and susceptibility to or mortality from COVID-19 infection. However, we observed a substantial increase in IL-6 and CRP levels associated with the severity of COVID-19, while no such trend was observed for IL-1β and TNF-α. This study showed a considerable rise in TNF-α and IL-1β serum levels exclusively in COVID-19 patients with TT rs2070788 TMPRSS2 SNP genotype compared to healthy controls. ConclusionIn this study conducted across multiple cities in Iran, no significant association was found between the TMPRSS2 rs2070788 SNP genotypes and COVID-19 severity or mortality.引言：宿主遗传变异已被证实为影响新冠病毒（COVID-19）感染的潜在危险因素。本研究旨在探讨伊朗人群中跨膜丝氨酸蛋白酶2（transmembrane serine protease type 2, TMPRSS2）rs2070788单核苷酸多态性（single nucleotide polymorphism, SNP）与新冠病毒感染预后的关联。 方法：本病例对照研究纳入伊朗境内756名新冠患者与59名健康个体。采用T-ARMS-PCR技术检测受试者的临床资料、血液样本以及跨膜丝氨酸蛋白酶2 rs2070788 G>A单核苷酸多态性的携带情况。此外，对采集的血液样本中的肿瘤坏死因子α（tumor necrosis factor α, TNF-α）、C反应蛋白（C-reactive protein, CRP）、白细胞介素6（interleukin-6, IL-6）及白细胞介素1β（IL-1β）血清水平进行了检测与评估。 结果：未发现跨膜丝氨酸蛋白酶2 rs2070788单核苷酸多态性的基因型与等位基因频率与新冠病毒感染易感性或病死率存在显著关联。不过，我们观察到IL-6与CRP水平随新冠病情严重程度升高而显著上升，而IL-1β与TNF-α未呈现此类变化趋势。本研究还发现，相较于健康对照人群，仅携带跨膜丝氨酸蛋白酶2 rs2070788 TT基因型的新冠患者，其血清TNF-α与IL-1β水平存在显著升高。 结论：在伊朗多城市开展的本研究中，未发现跨膜丝氨酸蛋白酶2 rs2070788单核苷酸多态性的基因型与新冠病情严重程度或病死率存在显著关联。