Mild food restriction and gene expression profile (liver). Rattus norvegicus

The aim of this study is to obtain the gene expression profiles of the liver of young growing rats after mild restriction of food intake for one week or one month. Overall design: Male Wistar rats, at 6 weeks of age, were fed with reduced diet (5%, 10%, 20% and 30% restriction) for one week or one month (n=5). After 16h fasting, the liver was then extracted and total RNA was isolated using an RNeasy mini kit with DNase treatment (QIAGEN). The RNA samples were pooled from five rats for each group. Then, DNA microarray analysis was performed using duplicate GeneChips (Affymetrix) on each pooled RNA sample.

本研究旨在获取轻度食物限饲1周或1个月后年轻生长大鼠的肝脏基因表达谱。
实验设计：选取6周龄的雄性Wistar大鼠，分别以5%、10%、20%、30%的限饲比例进行饲喂，持续1周或1个月，每组n=5。禁食16小时后摘取肝脏，使用QIAGEN公司的RNeasy微型试剂盒并结合DNase处理步骤提取总RNA。将每组5只大鼠的RNA样本混合后，对每份混合RNA样本采用复份Affymetrix GeneChips芯片进行DNA微阵列分析。