Oxford Nanopore Technologies MinION sequencing as a novel short amplicon metabarcoding tool

We tested the utility of Oxford Nanopore Technologies MinION long read sequencing for in situ short read amplicon sequencing of mitochondrial COI mini-barcodes. We identified host species and diet composition by sequencing droppings collected from five individual Irish brown long-eared bat (Plecotus auritus) roosts.

本研究评估了牛津纳米孔科技公司（Oxford Nanopore Technologies）MinION长读长测序技术应用于线粒体COI迷你条形码（mitochondrial COI mini-barcodes）的原位短读长扩增子测序的应用效能。研究团队通过对5个爱尔兰棕长耳蝠（Plecotus auritus）栖息巢点收集的粪便样本进行测序，鉴定了宿主物种及其饮食组成。