Confirmation of mating between Zygosacchromyces yeast stains by mating-type-like loci nucleotide sequences. Zygosaccharomyces sp. strain:NBRC1130, NBRC0740, NBRC1876

Whole genome sequences of NBRC0740 and NBRC1876 was determined by next generation sequencer HiSeq2500. Whole length of genome of Z. rouxii NBRC0740 is same to that of Z. rouxii type strains CBS732 (NBRC1130). On the other hand, whole length of genome of Zygosaccharomyces sp. NBRC1876 is twice as large as those of other stains. From this result, it is deduced that Zygosaccharomyces sp. NBRC1876 is natural interspecies hybrid between Z. rouxii and related species.

本研究利用下一代测序仪HiSeq2500完成了菌株NBRC0740与NBRC1876的全基因组测序。鲁氏接合酵母（Zygosaccharomyces rouxii）NBRC0740的基因组全长与鲁氏接合酵母模式菌株CBS732（NBRC1130）的基因组全长一致。另一方面，接合酵母属未定种（Zygosaccharomyces sp.）NBRC1876的基因组全长为其他菌株的两倍。基于上述结果，可推断接合酵母属未定种NBRC1876为鲁氏接合酵母与其近缘物种间形成的天然种间杂种。