Engineered Enucleated Mesenchymal Stem Cells Regulating Immune Microenvironment and Promoting Wound Healing

We conducted a transcriptomic analysis to evaluate DEK expression levels in the uninjured skin of healthy mice, wound tissue of healthy mice, and diabetic mice. Our findings revealed a significant upregulation of DEK in diabetic mouse wounds, with Gene Ontology (GO) analysis indicating enrichment in pathways related to neutrophil migration. Moreover, principal component analysis (PCA) results indicated that there are significant differences in gene expression between these two groups (Fig. 2D). Differential expression gene (DEG) analysis revealed 508 downregulated and 359 upregulated genes in the diabetic group compared with the health group. These downregulated genes are associated with skin, chondrocyte, and cartilage development, suggesting that the wound repair process is impaired in diabetic mice. Overall design: Comparative gene expression profiling analysis of RNA-seq data for skin tissue from uninjured skin of healthy mice, wound tissue of healthy mice, and diabetic mice.本研究通过转录组学分析，检测健康小鼠未损伤皮肤、健康小鼠创伤组织及糖尿病小鼠创伤组织中DEK的表达水平。研究结果显示，糖尿病小鼠创伤组织中DEK的表达显著上调；基因本体（Gene Ontology，GO）富集分析表明，差异表达基因富集于中性粒细胞迁移相关通路。此外，主成分分析（Principal Component Analysis，PCA）结果显示，两组样本的基因表达存在显著差异（图2D）。差异表达基因（Differential Expression Gene，DEG）分析显示，与健康对照组相比，糖尿病组共筛选得到508个下调基因与359个上调基因。上述下调基因与皮肤、软骨细胞及软骨发育密切相关，提示糖尿病小鼠的创伤修复过程存在受损情况。研究整体设计：针对健康小鼠未损伤皮肤、健康小鼠创伤组织及糖尿病小鼠创伤组织的RNA测序（RNA-seq）数据开展对比基因表达谱分析。