Genotyping of the F1 population derived by crossing Chardonnay and Tocai friulano to study the suscepitibility and resistance of Flavescence dorée in grapevine. Genotyping by sequencing of Chardonnay X Tocai friulano F1 population

“Flavescence dorée” (FD) occurs in many European viticulture regions, and it is considered one of the most destructive grapevine diseases. Understanding the genetic control of FD susceptibility is a crucial step in managing the disease. Identification of single nucleotide polymorphism (SNP) markers linked to FD resistance can be a powerful tool for the introgression of valuable genes needed to develop resistant varieties. Observations performed in many experimental vineyards revealed differences in resistance to FD among varieties, that appear to have a genetic basis. The cultivar Tocai friulano is less susceptible to FD, and its ability to compartmentalize FD phytoplasmas in the symptomatic portions of the plant was recently demonstrated (Casarin et al., 2023). Moreover, the comparison of the transcriptional changes between Tocai friulano and Chardonnay (highly susceptible) highlighted passive defence strategies in response to the insect vector and/or the phytoplasma infection (Bertazzon et al., 2019). The aim of this study was the genetic dissection of FD resistance revealed by Tocai friulano through the adoption of molecular approaches such as genotyping-by-sequencing (GBS). GBS was carried out on the F1 population derived by crossing Tocai friulano and Chardonnay.

金叶病（Flavescence dorée，FD）在欧洲诸多葡萄种植区均有发生，被认为是破坏性最强的葡萄病害之一。解析FD感病性的遗传调控机制是该病害防控的关键步骤。筛选与FD抗病性连锁的单核苷酸多态性（single nucleotide polymorphism，SNP）标记，可为培育抗病品种所需的优良基因渐渗提供有力工具。多项在试验葡萄园开展的观测结果显示，不同葡萄品种对FD的抗病性存在差异，且该差异具有遗传基础。托凯弗留拉诺（Tocai friulano）品种对FD的感病性较弱，近期研究已证实其可在植株症状部位限制FD植原体的扩散（Casarin等，2023）。此外，对比托凯弗留拉诺与高感病品种霞多丽（Chardonnay）的转录组变化，揭示了其针对昆虫媒介和/或植原体侵染的被动防御策略（Bertazzon等，2019）。本研究旨在通过测序分型（genotyping-by-sequencing，GBS）等分子手段，对托凯弗留拉诺所表现出的FD抗病性开展遗传解析。研究以托凯弗留拉诺与霞多丽杂交获得的F1分离群体为材料，完成了测序分型实验。