Disruption of Y chromosome expression in male lung cancer: replication

Lung cancer is the single most frequent cause of cancer death worldwide and is relatively more common in males. For reasons that are currently unknown, lung cancer is also associated with significantly worse outcomes in men than women. Here we replicate clustering of Y-chromosome transcripts identified previously as showing tumor-specific disruption in men with lung cancer. Human whole transcriptome data were generated from pulmonary tumor and, where possible, matched unaffected tissue (referred to hereon as ‘normal’) using the Affymetrix HuGene 1.1 ST microarray, giving a total of 123 samples from 69 patients. Tumor samples and adjacent normal lung tissue were donated from surgical resections, undertaken with curative intent.

肺癌是全球范围内最常见的癌症致死病因，且在男性群体中发病率相对更高。尽管具体关联机制尚不明确，临床数据显示男性肺癌患者的预后显著劣于女性患者。本研究对此前鉴定出的、在男性肺癌患者中呈现肿瘤特异性表达紊乱的Y染色体转录本进行了聚类验证。本研究采用Affymetrix HuGene 1.1 ST微阵列（Affymetrix HuGene 1.1 ST microarray），对肺肿瘤组织及可获取的配对正常肺组织（下文简称“正常组织”）开展全转录组检测，最终获得来自69名患者的共计123份样本的转录组数据。所有肿瘤组织及配对正常肺组织均来源于接受根治性手术切除的患者捐赠。