Orthologs of acyl-CoA synthetases and AcAS identified for phylogenetic analysis.

The full amino acid sequences of PfACS9, PfACS10, PfACS11, and PfAcAS, and the partial amino acid sequence of PfACS12 (the first 664 amino acids, excluding the thiamin-diphosphate-binding fold domain) were used as query sequence (green) in an iterative search (jackhmmer) (5 iterations) for orthologs in the UniProtKB database [160]. E-values found in our jackhmmr search are reported, and hits were identified as genes that showed an E-value of −50 for at least 1 query sequence. The E-value of genes that were below the E-value cutoff for a query sequence are indicated in red. The following genes were excluded from phylogenetic analysis: genes that were not identified as hits as the E-value was below the cutoff for all query sequences (gray shading), genes that did not align using the partial deletion cutoff of 50% in our alignment using ClustalW (dark gray shading), and genes that did not cluster with other genes in the phylogenetic tree (blue shading). (XLSX)