Nunu Dairy Microbiome Phage DM-1

With the advent of next-generation sequencing (NGS) on metagenomes, the elucidation of all genetic material from microbiomes has prompted a renewed interest towards uncultivated members of the virosphere. We describe the discovery of a novel phage from a metagenomic dataset on the West African fermented dairy product, nunu, with a custom bioinformatics workflow to potentially serve as a biocontrol agent against pathogenic <em>E. coli</em>. Initial dataset of ERR2014814 from NCBI was first subjected to Kraken2 to extract novel sequencing reads for further <em>de novo</em> assembly into contigs.

随着宏基因组（metagenome）下一代测序（NGS）技术的兴起，对微生物组（microbiome）全量遗传物质的解析工作，重新唤起了学界对病毒圈（virosphere）中未培养成员的研究兴趣。本研究从西非发酵乳制品nunu的宏基因组数据集中发现一株新型噬菌体，通过定制化生物信息学流程验证，该噬菌体有望作为致病性大肠杆菌（E. coli）的生物防治剂。研究首先对NCBI数据库中编号为ERR2014814的初始数据集采用Kraken2工具进行处理，以提取新型测序读段，随后开展从头组装（de novo assembly）得到重叠群（contigs）。