chimera of ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo fused to a proline-Rich-Domain (PRD) and profilin, bound to ADP-Mg-actin and a sulfate ion

chimera of ExoY Nucleotidyl Cyclase domain from Vibrio nigripulchritudo fused to a proline-Rich-Domain (PRD) and profilin, bound to ADP-Mg-actin and a sulfate ion Descriptor: 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ADENOSINE-5'-DIPHOSPHATE, Actin, ... Authors: Teixeira-Nunes, M, Renault, L, Retailleau, P. Deposit date: 2022-11-04 Release date: 2023-09-20 Last modified: 2023-10-04 Method: X-RAY DIFFRACTION (1.75 Å) Cite: Functional and structural insights into the multi-step activation and catalytic mechanism of bacterial ExoY nucleotidyl cyclase toxins bound to actin-profilin. Plos Pathog., 19, 2023

本数据集为来自黑色弧菌（Vibrio nigripulchritudo）的ExoY核苷酸环化酶结构域与富脯氨酸结构域（Proline-Rich-Domain, PRD）及前纤维蛋白（profilin）融合形成的嵌合蛋白，该蛋白结合了ADP-镁-肌动蛋白（ADP-Mg-actin）与一个硫酸根离子。描述项包含：2-氨基-2-羟甲基-1,3-丙二醇、腺苷-5'-二磷酸、肌动蛋白等。作者：Teixeira-Nunes, M、Renault, L、Retailleau, P。提交日期：2022-11-04，发布日期：2023-09-20，最后修改日期：2023-10-04。实验方法：X射线衍射（X-RAY DIFFRACTION，分辨率1.75 Å）。引用文献：《结合肌动蛋白-前纤维蛋白的细菌ExoY核苷酸环化酶毒素的多步激活与催化机制的功能与结构解析》，《公共科学图书馆·病原体（Plos Pathog.）》，19卷，2023年。