A multiOMICs approach shed light on the higher yield phenotype and abiotic stress tolerance in tobacco lines expressing the carrot lycopene β-cyclase1. A multiOMICs approach shed light on the higher yield phenotype and abiotic stress tolerance in tobacco lines expressing the carrot lycopene β-cyclase1

Recently we published a set of tobacco lines, expressing the Dacucus carota (carrot) DcLCYB1 gene, with accelerated development, increased carotenoid content, and plant yield. Due to this, expression of DcLCYB1 might be of general interest in crop species as a strategy to accelerate plant development and increase biomass production under adverse field conditions. However, to follow this path, a better understanding of the molecular basis of this phenotype is essential. Here, we combine OMICs (RNAseq, proteomics, metabolomics, and lipidomics) and stress experiments to advance our understanding on the broader effect of the LCYB expression on the tobacco genome and metabolism. Overall design: Overall 12 samples were analyzed including 3 transgenic lines and 1 wild type (control) and each sample consist of 3 biological replicates.

本团队近日发布了一组表达胡萝卜（Dacucus carota）来源DcLCYB1基因的烟草株系，该株系发育速率加快、类胡萝卜素含量提升且植株产量增加。鉴于此，在作物物种中表达DcLCYB1基因，或可作为一项在逆境田间条件下加速植株发育、提升生物量积累的通用策略，因此具备广泛的研究价值。然而，若要推进该策略的实际应用，深入解析该表型的分子基础至关重要。本研究整合组学（OMICs）分析（涵盖RNA测序（RNAseq）、蛋白质组学（proteomics）、代谢组学（metabolomics）与脂质组学（lipidomics））以及胁迫实验，以深化对LCYB基因表达对烟草基因组及代谢过程的广泛影响的认知。实验整体设计：本研究共分析12份样本，包含3个转基因株系与1个野生型（对照），每个样本设置3次生物学重复。