Oxford Nanopore MinION resistance-gene enrichment sequencing of Solanum americanum clone 954750186. Solanum americanum clone 954750186 Nanopore MinION RenSeq

Solanum americanum clone 954750186 resistance-gene enrichment sequencing (RenSeq) was performed using four Oxford Nanopore MinION flowcells (R7.3 chemistry). The data was assembled using Canu-1.0 and further corrected using nanopolish-0.4.0 and Pilon-1.18. The Oxford Nanopore MinION data was compared to PacBio data described in 'Witek K, Jupe F, Witek AI, Baker D, Clark MD, Jones JDG. Accelerated cloning of a potato late blight–resistance gene using RenSeq and SMRT sequencing. Nature Biotechnology. 2016;34:656–60.'. For comparison the PacBio data was assembled using Canu-1.0 and HGAP.

美洲龙葵（Solanum americanum）克隆株系954750186的抗病基因富集测序（Resistance-gene enrichment sequencing, RenSeq）实验，采用4张牛津纳米孔（Oxford Nanopore）MinION测序流通池（配套R7.3化学试剂）开展。所得测序数据使用Canu-1.0进行组装，并通过nanopolish-0.4.0与Pilon-1.18完成后续校正。将该牛津纳米孔MinION测序数据与报道于文献的太平洋生物科学（PacBio）测序数据进行比对，所涉文献为：Witek K, Jupe F, Witek AI, Baker D, Clark MD, Jones JDG. 利用RenSeq与单分子实时测序（Single Molecule Real-Time sequencing, SMRT）技术快速克隆马铃薯晚疫病抗病基因. 《自然·生物技术》, 2016, 34: 656–60. 为完成本次比对，该PacBio测序数据通过Canu-1.0与分层基因组组装流程（HGAP）完成组装。